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© Bernd Degen
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Institute of

FG Forest Genetics

Dr. Tobias Brügmann

Institute of Forest Genetics

Sieker Landstraße 2
22927 Großhansdorf
Telephone
+49 4102 696 170
Fax
+49 4102 696 200
Email
tobias.bruegmann@thuenen.de

Head of Junior Research Group "Genetic Technologies"

Scientific projects at the Thünen Institute

Since 2021: Head of the Junior Research Group "Genetic Technologies"

2018-2021: Project Scientist in "aProPop" (Development of a DNA-free genome editing system in poplar by transient Cas9/gRNA plasmid and ribonucleinprotein transfer into protoplasts and germinating pollen)

2012-2017: Project Scientist at the Thünen Institute of Forest Genetics in the project "PopMass" (Development and use of novel gene technologies to increase biomass yield in the woody perennial Populus spec.)

The Junior Research Group is funded by the Federal Ministry of Food and Agriculture (BMEL) via Fachagentur für Nachwachsende Rohstoffe (FNR).

All other projects were funded by the Federal Federal Ministry of Education and Research (BMBF).

CV

Promotion 2017: "Genetische Modifikation von SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), FRUITFULL (FUL) und weiterer Kandidatengene in Pappelhybriden (Populus spec.)" (final grade "mit Auszeichnung/summa cum laude"; supervisors PD Dr. M. Fladung & Prof. Dr. H.-P. Mühlbach, co-supervisor PD Dr. D. Warnecke)

Since 2011: Master of Science, Biology (Molecular Biology and Biotechnology)

2011: Master thesis "Entwicklung molekularer Marker zur genetischen Charakterisierung unterschiedlicher Genotypen der Gattung Populus" ("Development of molecular markers for genetic characterization of different genotypes from the genus Populus")

2009: Bachelor thesis "Optimierung von RNA-Extraktion und cDNA-Synthese in Quercus robur" ("Optimization of RNA extraction and cDNA synthesis for Quercus robur")

2006-2011: Study of biology, University of Hamburg, Germany

Supervised Theses

Bachelor Thesis Alina Fomin (2022/2023)
Course Biochemistry, University of Lübeck, Lübeck

Bachelor Thesis Keelin Meyer (2022/2023)
Course Biotechnology, Hochschule Emden-Leer, Emden

Bachelor Thesis Thalia von Nethen (2021/2022)
Course Biotechnology, Hochschule Emden-Leer, Emden

Bachelor Thesis Stefan Zebbedies (2020/2021)
Course Technology of Renewable Resources, Hochschule Hannover, Hannover

Bachelor Thesis Oliver Sulkowski (2019/2020)
Course Bioinformatics/Biotechnology, Hochschule Emden-Leer, Emden

Bachelor Thesis Jakob Fromme (2017)
Course Biotechnology, Hochschule für angewandte Wissenschaften, Hamburg

Publications

  1. 0

    Riefler M, Brügmann T, Fladung M, Schmülling T (2022) A constitutively active cytokinin receptor variant increases cambial activity and stem growth in poplar. Int J Mol Sci 23(15):8321, DOI:10.3390/ijms23158321

    https://literatur.thuenen.de/digbib_extern/dn065121.pdf

  2. 1

    Zahn V, Sievers A-J, Fladung M, Brügmann T (2022) A reliable in vitro culture system for Fagus sylvatica - first steps to expend the scope of genome editing in forest trees to beech. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 249

  3. 2

    Zahn V, Brügmann T, Fladung M (2022) Combining bacterial and viral elements for efficient gene targeting in poplar. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 85

  4. 3

    Zahn V, Fladung M, Brügmann T (2022) Combining bacterial and viral elements for efficient gene targeting in poplar. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 24

  5. 4

    Brügmann T, Zebbedies S, Zahn V, Fladung M (2022) Diverse mutation patterns include large deletions in a CRISPR/nCas9 double nicking approach poplar. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 48

  6. 5

    Brügmann T, Zebbedies S, Zahn V, Fladung M (2022) Diverse mutation patterns include large deletions in a CRISPR/nCas9 double nicking approach poplar. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 248

  7. 6

    Brügmann T, Fladung M, Schröder H (2022) Flexible DNA isolation procedure for different tree species as a convenient lab routine. Silvae Genetica 71:20-30, DOI:10.2478/sg-2022-0003

    https://literatur.thuenen.de/digbib_extern/dn065030.pdf

  8. 7

    Fendel A, Fladung M, Brügmann T (2022) Improvement of drought stress tolerance in poplars (Populus) by modification of candidate genes. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 35

  9. 8

    Fendel A, Fladung M, Brügmann T (2022) Improvement of drought stress tolerance in poplars (Populus) by modification of candidate genes. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 104

  10. 9

    Brügmann T, Zahn V, Fendel A, Zebbedies S, Sievers A-J, Becker D, Fladung M (2022) Neue biotechnologische Methoden für Gehölze. In: Liesebach M, Tröber U (eds) 7. Tagung der Sektion Forstgenetik/Forstpflanzenzüchtung : "Beiträge von Forstpflanzenzüchtung und Forstgenetik für den Wald von Morgen" ; Ahrensburg, 12. bis 14. September 2022, Abstract-Band und Exkursionsführer. p 11

  11. 10

    Brügmann T, Biricolti S, Becker D, Fladung M (2022) On track towards DNA-free genome editing. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 49

  12. 11

    Brügmann T, Deecke K, Fladung M (2019) Evaluating the efficiency of gRNAs in CRISPR/Cas9 mediated genome editing in poplars. Int J Mol Sci 20(15):3623, DOI:10.3390/ijms20153623

    https://literatur.thuenen.de/digbib_extern/dn061163.pdf

  13. 12

    Brügmann T, Fladung M (2019) Genom-Editierung in Bäumen. AFZ Der Wald 74(5):16-18

    https://literatur.thuenen.de/digbib_extern/dn060910.pdf

  14. 13

    Brügmann T, Wetzel H, Hettrich K, Smeds A, Willför S, Kersten B, Fladung M (2019) Knockdown of PCBER1, a gene of neolignan biosynthesis, resulted in increased poplar growth. Planta 249(2):515-525, DOI:10.1007/s00425-018-3021-8

  15. 14

    Brügmann T, Fladung M (2019) Overexpression of both flowering time genes AtSOC1 and SaFUL revealed huge influence onto plant habitus in poplar. Tree Genetics Genomes 15:20, DOI:10.1007/s11295-019-1326-9

  16. 15

    Brügmann T, Polak O, Deecke K, Nietsch J, Fladung M (2019) Poplar transformation. Meth Mol Biol 1864:165-177

  17. 16

    Brügmann T, Fladung M (2016) Flowering time genes influence biomass production in poplars. In: Hensel G (ed) Annual meeting : new breeding technologies in times of politically enforces research prohibition ; German Society of Plant Biotechnology e.V. ; book of abstracts ; Gatersleben, 02.-04.05.2016. Gatersleben: Gesellschaft für Pflanzenbiotechnologie, p 23

  18. 17

    Brügmann T, Polak O, Fladung M (2015) Development and use of novel gene technologies to increase biomass yield in the woody perennial Populus spec. (PopMass) : project A - modification of genes expressed in xylem and flower. In: Conference documents Plant 2040 Status Seminar, March 4 - 6, 2015 in Potsdam. pp 67-68

  19. 18

    Brügmann T, Fladung M (2015) Genetic approaches to increase biomass yield in the woody perennial Populus. In: Vettori C, Vendramin GG, Paffetti D, Travaglini D (eds) IUFRO Tree Biotechnology 2015 Conference : "Forests: the importance to the planet and society" ; 8-12 June 2015, Florence, Italy ; Proceedings. Florenz: IUFRO, pp 507-509

  20. 19

    Schröder H, Brügmann T, Fladung M (2015) Nuclear and chloroplast SNP markers support successful poplar breeding. In: Vettori C, Vendramin GG, Paffetti D, Travaglini D (eds) IUFRO Tree Biotechnology 2015 Conference : "Forests: the importance to the planet and society" ; 8-12 June 2015, Florence, Italy ; Proceedings. Florenz: IUFRO, pp 295-296

  21. 20

    Hönicka H, Fladung M, Lehnhardt D, Brügmann T (2013) Pleiotropic effects of "flowering" genes in transgenic poplar. In: SPP1530 workshop: Pleiotropic effects of flowering time genes and impact on adaptation and speciation, January 21-23,2013, Max Planck Institute for Plant Breeding Research, Köln. p 13

  22. 21

    Brügmann T, Fladung M (2013) Potentials and limitations of the cross-species transfer of nuclear microsatellite marker in six species belonging to three sections of the genus PopulusL.. Tree Genetics Genomes 9(6):1413-1421, DOI:10.1007/s11295-013-0647-3

  23. 22

    Brügmann T (2011) Entwicklung molekularer Marker zur genetischen Charakterisierung von Genotypen verschiedener Arten der Gattung Populus L.. Hamburg: Universität, 134 p, Hamburg, Univ, Fachber Biologie, Masterarbeit, 2011

  24. 23

    Brügmann T (2009) Optimierung von RNA-Extraktion und cDNA-Synthese aus Quercus robur. Hamburg: Univ, 76 p, Hamburg, Univ, Department Biologie, Bachelorarbeit, 2009

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