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Institute of

FG Forest Genetics

Project

Genome analysis in trees


Lead Institute FG Institute of Forest Genetics

Structural and functional analysis of genomes of forest trees

Trees are characterized by a number of specific characteristics which are under genetic control, like longevity and wood formation.

Background and Objective

It is expected that several genes are involved in the mentioned processes. The aim of this task is the isolation and characterization of regulatory and other different gene sequences which are specific for trees. Species of the genus Populus (poplar) will be used for the investigations.

Approach

Poplars are an excepted model system for trees because of their relatively small genome and accessibility to the Agrobacterium transformation system. Following Arabidopsis and rice the genome of Populus has been totally sequenced. These information serve as basis for the planned investigations.

Preliminary Results

The strategy is based on results of earlier work. Here it was shown for the first time that the genetic transformation of poplar using the transposon Ac from maize is feasible. The results show that Ac excises from its original position in the construct and re-integrates somewhere in the genome (transposition). Further it was demonstrated, that transposition of Ac still occurs in six-years old transgenic plants.; ; Following transformation of Ac more than 100 different, independent transgenic aspe

Publications

  1. 0

    Mosca E, Cruz F, Gomez-Garrido J, Bianco L, Rellstab C, Brodbeck S, Csillery K, Fady B, Fladung M, Fussi B, Gömöry D, Gonzalez-Martinez SC, Grivet D, Gut M, Hansen OK, Heer K, Kaya Z, Krutovsky KV, Kersten B, Liepelt S, et al (2019) A reference genome sequence for the European silver fir (Abies alba Mill.): A community-generated genomic resource. G3 Genes Genomes Genetics 9(7):2039-2049, DOI:10.1534/g3.119.400083

    https://literatur.thuenen.de/digbib_extern/dn061097.pdf

  2. 1

    Hernandez-Velasco J, Hernández-Diaz JC, Fladung M, Cañadas-Lopez A, Prieto-Ruiz JA, Wehenkel C (2017) Spatial genetic structure in four Pinus species in the Sierra Madre Occidental, Durango, Mexico. Can J Forest Res 47:73-80, DOI:10.1139/cjfr-2016-0154

  3. 2

    Ortiz-Olivas ME, Hernández-Diaz JC, Fladung M, Cañadas-Lopez A, Prieto-Ruiz JA, Wehenkel C (2017) Spatial genetic structure within and among seed stands of Pinus engelmannii Carr. and Pinus leiophylla Schiede ex Schltdl. & Cham, in Durango, Mexico. Forests 8:22, DOI:10.3390/f8010022

    https://literatur.thuenen.de/digbib_extern/dn058175.pdf

  4. 3

    Kersten B, Faivre Rampant P, Mader M, Le Paslier M-C, Bounon R, Berard A, Vettori C, Schröder H, Leplé J-C, Fladung M (2016) Genome sequences of Populus tremula chloroplast and mitochondrion: Implications for holistic poplar breeding. PLoS One 11(1):e0147209, DOI:10.1371/journal.pone.0147209

    https://literatur.thuenen.de/digbib_extern/dn056232.pdf

  5. 4

    Mader M, Le Paslier M-C, Bounon R, Berard A, Faivre Rampant P, Fladung M, Leplé J-C, Kersten B (2016) Whole-genome draft assembly of Populus tremula x P. alba clone INRA 717-1B4. Silvae Genetica 65(2):74-79, DOI:10.1515/sg-2016-0019

    https://literatur.thuenen.de/digbib_extern/dn059214.pdf

  6. 5

    Pakull B, Kersten B, Lüneburg J, Fladung M (2015) A simple PCR-based marker to determine sex in aspen. Plant Biol 17(1):256-261, DOI:10.1111/plb.12217

  7. 6

    Kersten B, Voß M-M, Fladung M (2015) Development of mitochondrial SNP markers in different Populus species. Trees 29(2):575-582, DOI:10.1007/s00468-014-1136-5

  8. 7

    Fladung M (2014) Prospects of using a modified Ac/Ds transposon system from maize for activation tagging in the tree species Populus. In: Ramawat KG, Mérillon J-M, Ahuja MR (eds) Tree biotechnology. Boca Raton: CRC Press ; Taylor & Francis, pp 469-482

  9. 8

    Kersten B, Pakull B, Groppe K, Lüneburg J, Fladung M (2014) The sex-linked region in Populus tremuloides Turesson 141 corresponds to a pericentromeric region of about two million base pairs on P. trichocarpa chromosome 19. Plant Biol 16(2):411-418, DOI:10.1111/plb.12048

  10. 9

    Fladung M (2013) Efficient in vitro plantlet regeneration in Populus euphrata Oliver [online]. Afr J Biotechnol 12(8):826-832, zu finden in <http://www.academicjournals.org/journal/AJB/article-full-text-pdf/81F997626646> [zitiert am 12.11.2013]

  11. 10

    Fladung M, Gebhardt K, Kersten B (2013) Erbgut verschiedener Nadelbäume vollständig entschlüsselt. AFZ Der Wald 68(20):13-15

    https://literatur.thuenen.de/digbib_extern/dn052682.pdf

  12. 11

    Fladung M, Hönicka H, Ahuja MR (2013) Genomic stability and long-term transgene expression in poplar. Transgenic Res 22(6):1167-1178, DOI:10.1007/s11248-013-9719-2

  13. 12

    Bubner B, Fladung M, Lentzsch P, Münzenberger B, Hüttl RF (2013) Individual tree genotypes do not explain ectomycorrhizal biodiversity in soil cores of a pure stand of beech (Fagus sylvatica L.). Trees 27(5):1327-1338, DOI:10.1007/s00468-013-0881-1

  14. 13

    Kersten B, Ghirardo A, Schnitzler JP, Kanawati B, Schmitt-Kopplin P, Fladung M, Schröder H (2013) Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.. BMC Genomics 14:737, DOI:10.1186/1471-2164-14-737

    https://literatur.thuenen.de/digbib_extern/dn052678.pdf

  15. 14

    Höltken AM, Fladung M, Streckenbach M, Dujesiefken D (2013) Möglichkeiten DNA-basierter Methoden für Baumgutachten. Jb Baumpflege 2013:19-25

  16. 15

    Brügmann T, Fladung M (2013) Potentials and limitations of the cross-species transfer of nuclear microsatellite marker in six species belonging to three sections of the genus PopulusL.. Tree Genetics Genomes 9(6):1413-1421, DOI:10.1007/s11295-013-0647-3

  17. 16

    Grima-Pettenati J, Leplé J-C, Gion JM, Harvengt L, Fladung M, Kamm B, Pinto Paiva JA, Rodrigues JC, Costa Leal L, Canton FR, Gallardo F, Allona I, Sixto H, Ruiz F (2013) TreeForJoules - supporting sustainable second generation biofuels. In: International innovation : dissemination science, research and technology . Auzeville: Universite Paul Sabatier, p 2

  18. 17

    Fladung M, Polak O (2012) Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery. BMC Genomics 13(61), DOI:10.1186/1471-2164-13-61

    https://literatur.thuenen.de/digbib_extern/dn050044.pdf

  19. 18

    Schröder H, Wühlisch G von, Fladung M (2012) Auch bei Pappeln ist nicht immer drin, was drauf steht. AFZ Der Wald 67(5):13-15

    https://literatur.thuenen.de/digbib_extern/dn050075.pdf

  20. 19

    Höltken AM, Schröder H, Wischnewski N, Degen B, Magel EA, Fladung M (2012) Development of DNA-based methods to identify CITES-protected timber species: a case study in the Meliaceae family. Holzforsch 66(1):97-104, DOI:10.1515/HF.2011.142

  21. 20

    Schröder H, Höltken AM, Fladung M (2012) Differentiation of Populus species using chloroplast single nucleotide polymorphism (SNP) markers – essential for comprehensible and reliable poplar breeding. Plant Biol 14(2):374-381, doi:10.1111/j.1438-8677.2011.00502.x

  22. 21

    Fladung M (2012) Entwicklung und Nutzung neuartiger genetischer Technologien zur Erhöhung von Biomasseerträgen in Populus spec. (PopMass). Beitr Nordwestdt Forstl Versuchsanst 8:

  23. 22

    Schröder H, Fladung M (2012) Identifizierung kommerziell genutzter Pappelklone - der Nutzen molekularer Marker für die Züchtung. Beitr Nordwestdt Forstl Versuchsanst 8:257-265

  24. 23

    Kersten B, Pakull B, Fladung M (2012) Mapping of the sex trait and sequence analysis of two linked genomic regions in Populus tremuloides. ScienceMed 3(3):203-210

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