Head of Unit

PD Dr. Matthias Fladung

Sieker Landstraße 2

22927 Großhansdorf

Telefon +49 4102 696 107

Fax +49 4102 696 200

e-Mail matthias.fladung@  thuenen.de

SNP-Pappel

Project

SNP-diagnosis of breeding-relevant properties in Salicaceae

With the availability of the annotated genome sequence of Populus trichocarpa and the very large collections of EST sequences, the challenge today is to describe breeding and breeding strategies at the level of genes or even to describe below.

Background and Objective

One challenge today is to describe the variability of gene expression with nucleotide diversity. The objectives of this project are therefore to evaluate the nucleotide diversity in for breeding relevant clones and hybrids; to develop a SNP database of poplar for at least 20 candidate genes for breeding-related properties; and comparative analysis of genes with the highest SNP polymorphisms for quantitative traits.

Approach

One possibility for SNP diagnosis of breeding-relevant properties is the so-called candidate gene approach. Here, genes are a priori selected as possible candidate genes for drought tolerance, wound-/disease resistance, rooting ability, biomass production (CO2 fixation), lignification, etc. and the variation on the DNA level in different individuals and clones is determined. Therefore, in this project at least 20 candidate genes will be analyzed in different poplar clones to determine nucleotide diversity and establish a SNP database.

The expected outcome from the project will provide important clues about the commercial applicability of SMART-Breeding optimized poplar genotypes. The scientific prospects are very high because fundamental knowledge about the relationship between the SNP patterns and expression of plant features are expected.

Results

One possibility for SNP diagnosis of breeding-relevant properties is the so-called candidate gene approach. Here, genes are a priori selected as possible candidate genes for drought tolerance, wound-/disease resistance, rooting ability, biomass production (CO2 fixation), lignification, etc. and the variation on the DNA level in different individuals and clones is determined. Therefore, in this project at least 20 candidate genes will be analyzed in different poplar clones to determine nucleotide

Thünen-Contact


Involved Thünen-Partners


Involved external Thünen-Partners


Funding Body

  • Federal Ministry of Food und Agriculture (BMEL)
    (national, öffentlich)

Duration

6.2010 - 5.2013

More Information

Projekt type:
Project status: finished

Publications

hits: 4

  1. Kersten B, Faivre Rampant P, Mader M, Le Paslier M-C, Bounon R, Berard A, Vettori C, Schröder H, Leplé J-C, Fladung M (2016) Genome sequences of Populus tremula chloroplast and mitochondrion: Implications for holistic poplar breeding. PLoS One 11(1):e0147209, DOI:10.1371/journal.pone.0147209
    pdf document (limited accessibility) 1192 kb
  2. Höltken AM, Fladung M, Streckenbach M, Dujesiefken D (2013) Möglichkeiten DNA-basierter Methoden für Baumgutachten. Jb Baumpflege 2013:19-25
  3. Brügmann T, Fladung M (2013) Potentials and limitations of the cross-species transfer of nuclear microsatellite marker in six species belonging to three sections of the genus Populus L.. Tree Genetics Genomes 9(6):1413-1421, doi:10.1007/s11295-013-0647-3
  4. Pfennig K, Hoffmann M, Brauer M, Liepelt S, Fladung M, Gebhardt K (2012) SNP - Diagnose züchtungsrelevanter Eigenschaften von Salicaceaen. Beitr Nordwestdt Forstl Versuchsanst 8:379-380