Head of Unit

Dr. Mirko Liesebach

Sieker Landstraße 2

22927 Großhansdorf

Telefon +49 4102 696 156

Fax +49 4102 696 200

e-Mail: mirko.liesebach@  thuenen.de

Poplar genotyping



Genetic identification in the genus Populus L. with nuclear microsatellite markers

The increasing interest in cultivation of poplars as an energy crop for biomass production actually requires much effort in breeding programmes, especially for varieties suitable for growth in short rotation systems.

Background and Objective

Existing clone collections and offspring material growing in field trials are composed of members of the sections Tacamahaca (balsam poplars), Aigeiros (black poplars, cottonwoods) and Populus (white poplars and aspens) and intersectional hybrids between black and balsam poplars. The application of genetic markers is a very important tool for the identification of clones in poplar and aspen breeding, as morphological traits are not sufficient for that purpose for closely related material. Furthermore, it can support the taxonomic classification in case of doubt.


Nuclear microsatellite loci are well-established
markers for clone identification and paternity
tests and are effective in poplars as well. They provide reliable results for a genetic characterisation
of parents and their offspring individuals. Additional taxonomic information can be derived from single informative markers as well as from multivariate analyses.

Links and Downloads


Involved Thünen-Partners


9.2009 - 6.2019

More Information

Projekt type:
Project status: ongoing


hits: 8

  1. Belyaeva IV, Dutton C, Govaerts RHA, Liesebach H, McGinn K, Steenackers M, Taylor G, Pickett J (2020) Verification of names for certain Populus L. clones (Salicaceae) commonly grown in the United Kingdom. Skvortsovia 6(3):87-116
  2. Liesebach H, Liesebach M (2016) Züchtung von Aspen und Hybridaspen für den Kurzumtrieb - Genotypisierung auf Versuchsflächen zum Nachweis von Wurzelbrut. J Kulturpfl 68(1):1-6, DOI:10.5073/JFK.2016.01.01
  3. Ulrich K, Liesebach H, Ewald D (2015) Erzeugung, Nutzung und genetische Charakterisierung polyploider Pappeln. Thünen Rep 26:98-120
    pdf document (limited accessibility) 1271 kb
  4. Liesebach H, Ulrich K, Ewald D (2015) FDR and SDR processes in meiosis and diploid gamete formation in poplars (Populus L.) detected by centromere-associated microsatellite markers. Tree Genetics Genomes 11:801, DOI:10.1007/s11295-014-0801-6
  5. Ewald D, Ulrich K, Liesebach H (2012) Erzeugung triploider Individuen und intersektioneller Hybriden bei verschiedenen Pappelarten. Beitr Nordwestdt Forstl Versuchsanst 8:181-193
  6. Liesebach H, Schneck V, Ewald E (2011) Klonidentifizierung in der Gattung Populus L. mit nuklearen Mikrosatellitenmarkern. Mitt Forschungsanst Waldökologie Forstwirtsch 69/11:115-122
  7. Liesebach H, Naujoks G, Ewald D (2011) Successful hybridisation of normally incompatible hybrid aspen (Populus tremula x P. tremuloides) and eastern cottonwood (P. deltoides). Sex Plant Reprod 24(3):189-198, doi:10.1007/s00497-010-0156-6
  8. Liesebach H, Schneck V, Ewald E (2010) Clonal fingerprinting in the genus Populus L. by nuclear microsatellite loci regarding differences between sections, species and hybrids. Tree Genetics Genomes 6(2):259-269, DOI:10.1007/s11295-009-0246-5