![[Translate to English:]](/media/_processed_/f/3/csm_2022_Titelbild_gross2_Saatgut_in_Hand_9ffb8f5748.jpg "[Translate to English:]")
![[Translate to English:]](/media/_processed_/f/3/csm_2022_Titelbild_gross2_Saatgut_in_Hand_c17270fcc0.jpg "[Translate to English:]")
Dr. Tobias Brügmann
Institute of Forest Genetics
Sieker Landstraße 222927 Großhansdorf
- Telephone
- +49 4102 696 170
- Fax
- +49 4102 696 200
- tobias.bruegmann@thuenen.de
Head of Junior Research Group "Genetic Technologies"
Scientific projects at the Thünen Institute
Since 2021: Head of the Junior Research Group "Genetic Technologies"
2018-2021: Project Scientist in "aProPop" (Development of a DNA-free genome editing system in poplar by transient Cas9/gRNA plasmid and ribonucleinprotein transfer into protoplasts and germinating pollen)
2012-2017: Project Scientist at the Thünen Institute of Forest Genetics in the project "PopMass" (Development and use of novel gene technologies to increase biomass yield in the woody perennial Populus spec.)
The Junior Research Group is funded by the Federal Ministry of Food and Agriculture (BMEL) via Fachagentur für Nachwachsende Rohstoffe (FNR).
All other projects were funded by the Federal Federal Ministry of Education and Research (BMBF).
CV
Promotion 2017: "Genetische Modifikation von SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), FRUITFULL (FUL) und weiterer Kandidatengene in Pappelhybriden (Populus spec.)" (final grade "mit Auszeichnung/summa cum laude"; supervisors PD Dr. M. Fladung & Prof. Dr. H.-P. Mühlbach, co-supervisor PD Dr. D. Warnecke)
Since 2011: Master of Science, Biology (Molecular Biology and Biotechnology)
2011: Master thesis "Entwicklung molekularer Marker zur genetischen Charakterisierung unterschiedlicher Genotypen der Gattung Populus" ("Development of molecular markers for genetic characterization of different genotypes from the genus Populus")
2009: Bachelor thesis "Optimierung von RNA-Extraktion und cDNA-Synthese in Quercus robur" ("Optimization of RNA extraction and cDNA synthesis for Quercus robur")
2006-2011: Study of biology, University of Hamburg, Germany
University Teaching
and further responsibilities
Lecturer at the University of Hamburg
- Summer semester 2025: Seminar "Plant Biotechnology" in the Master's degree programme in Biology
- Winter semester 2023/24: "Molecular Biology and Genetics" practical course in the Bachelor's degree programme in Biology Education
- Winter semester 2023/24: Lecture "Plant Biotechnology" in the Master's programme in Biology
- Summer semester 2023: Seminar "Plant Biotechnology" in the Master's degree programme in Biology
- Summer semester 2022: "Molecular Biology and Genetics" practical course in the Bachelor's degree programme in Biology
Core Group member of the EU COST-Action "CopyTree", Webinar Coordinator
Certificate of competence for project managers and persons responsible for biological safety according to §15 Gentechnik-Sicherheitsverordnung (GenTSV)
Master Thesis Emily Idahl (2025)
Course Biology, University of Hamburg
Bachelor Thesis Amelie Keller (2025)
Course Biology Education, University of Hamburg
Bachelor Thesis Luisa Bisping (2025)
Course Biology Education, University of Hamburg
Bachelor Thesis Godje Roß (2025)
Course Biology, University of Hamburg
Master Thesis Sarah Wodtke (2024)
Course Biology, University of Hamburg
Master Thesis Anke Bollen (2023/2024)
Course Applied Environmental Sciences, University of Koblenz
Master Thesis Felix Wiedemann (2023/2024)
Course Biotechnology, University of Hannover
Master Thesis Friederike Schwarzer (2023/2024)
Course Bioinformatics, TU Braunschweig
Bachelor Thesis Ina Martini (2023)
Course Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Alina Fomin (2022/2023)
Course Biochemistry, University of Lübeck
Bachelor Thesis Keelin Meyer (2022/2023)
Course Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Thalia von Nethen (2021/2022)
Course Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Stefan Zebbedies (2020/2021)
Course Technology of Renewable Resources, Hochschule Hannover
Bachelor Thesis Oliver Sulkowski (2019/2020)
Course Bioinformatics/Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Jakob Fromme (2017)
Course Biotechnology, Hochschule für angewandte Wissenschaften
Publications
- 0
Zahn V, Fendel A, Sievers A-J, Fladung M, Brügmann T (2025) Benefiting from the past: establishing in vitro culture of European beech (Fagus sylvatica L.) from provenance trial trees and seedlings. Plant Methods 21:31, DOI:10.1186/s13007-025-01350-3
- 1
Brügmann T, Zahn V, Fendel A, Fladung M (2025) Biotechnologische Innovationen an Rotbuchen und weiteren Baumarten. Thünen Rep 119:211-216, DOI:10.3220/253-2025-23
- 2
Wodtke S, Fendel A, Brügmann T (2025) Brush them away : a simple method to remove trichomes and visualize stomata. Mar 24, 2025. Berkeley: protocols io, DOI:10.17504/protocols.io.kxygxwjqwv8j/v1
- 3
Grünhofer P, Herzig L, Zhang Q, Vitt S, Stöcker T, Malkowsky Y, Brügmann T, Fladung M, Schreiber L (2024) Changes in wax composition but not amount enhance cuticular transpiration. Plant Cell Environ 47(1):91-105, DOI:10.1111/pce.14719
- 4
Brügmann T, Fendel A, Zahn V, Fladung M (2024) Genome editing in forest trees. In: Ricroch A, Eriksson D, Miladinovic D, Sweet JB, Laere K van, Wozniak-Gientka E (eds) A roadmap for plant genome editing. Cham: Springer, pp 347-372, DOI:10.1007/978-3-031-46150-7_20
- 5
Brügmann T, Fladung M (2024) Genscheren in der Forstpflanzenzüchtung? AFZ Der Wald 79(5):27-30
- 6
Brügmann T, Orgel F, Jelkmann S, Schröder H (2024) Isolation of high quality RNA from tree leaves using Polyclar in the Spectrum Plant Total RNA Kit. Oct 08, 2024. Berkeley: protocols io, DOI:10.17504/protocols.io.14egn6qkml5d/v1
- 7
Rupps A, Thiesen F, Raschke J, Walther M, Ehmke E, Safranek L, Eckardt J, Schmidt A, Beck W, Bubner B, Eisold A-M, Zahn V, Fendel A, Brügmann T (2024) PInK-NET: Resuming the network idea of in vitro tree labs in Germany. In: Sota V, Werbrouck S (eds) In vitro culture of woody crops: problem solving by new approaches : The 2nd Conference of Cost Action CA21157 CopyTree, 22-24 April, 2024, Bulduri Technical School, Jurmala, Latvia ; Book of proceedings. pp 221-230
- 8
Cardi T, Murovec J, Bakhsh A, Boniecka J, Brügmann T, Bull SE, Eeckhaut T, Fladung M, Galovic V, Linkiewicz A, Lukan T, Mafra I, Michalski K, Kavas M, Nicolia A, Nowakowska J, Sagi L, Sarmiento C, Yildirim K, Zlatkovic M, et al (2023) CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation. Trends Plant Sci 28(10):1144-1165, DOI:10.1016/j.tplants.2023.05.012
- 9
Shahzaib M, Brügmann T, Shakeel M, Khan SH, Azhar MT, Atif RM, Fladung M, Rana IA (2023) Development of climate smart fruit plants via CRISPR/Cas genome editing systems: A spatiotemporal review [Preprint]. 2023051887. Basel: Preprintsorg, 22 p, DOI:10.20944/preprints202305.1887.v1
- 10
Zahn V, Sievers A-J, Fladung M, Brügmann T (2023) Etablierung einer stabilen in vitro-Kultur für Fagus sylvatica - Ein erster Schritt hin zur Genomeditierung in Buchen. Thünen Rep 105:9-10
- 11
Fendel A, Brügmann T (2023) Kontrolliert unter Stress. AFZ Der Wald 78(20):12-15
- 12
Brügmann T, Zahn V, Fendel A, Zebbedies S, Sievers A-J, Becker D, Fladung M (2023) Neue biotechnologische Methoden für Gehölze. Thünen Rep 105:6-8
- 13
Fendel A, Fladung M, Brügmann T (2023) Steigerung der Trockenstresstoleranz in Bäumen durch genetische Modifikationen. Thünen Rep 105:88-89
- 14
Riefler M, Brügmann T, Fladung M, Schmülling T (2022) A constitutively active cytokinin receptor variant increases cambial activity and stem growth in poplar. Int J Mol Sci 23(15):8321, DOI:10.3390/ijms23158321
- 15
Zahn V, Sievers A-J, Fladung M, Brügmann T (2022) A reliable in vitro culture system for Fagus sylvatica - first steps to expend the scope of genome editing in forest trees to beech. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 249
- 16
Zahn V, Brügmann T, Fladung M (2022) Combining bacterial and viral elements for efficient gene targeting in poplar. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 85
- 17
Zahn V, Fladung M, Brügmann T (2022) Combining bacterial and viral elements for efficient gene targeting in poplar. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 24
- 18
Brügmann T, Zebbedies S, Zahn V, Fladung M (2022) Diverse mutation patterns include large deletions in a CRISPR/nCas9 double nicking approach poplar. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 48
- 19
Brügmann T, Zebbedies S, Zahn V, Fladung M (2022) Diverse mutation patterns include large deletions in a CRISPR/nCas9 double nicking approach poplar. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 248
- 20
Brügmann T, Fladung M, Schröder H (2022) Flexible DNA isolation procedure for different tree species as a convenient lab routine. Silvae Genetica 71:20-30, DOI:10.2478/sg-2022-0003
- 21
Fendel A, Fladung M, Brügmann T (2022) Improvement of drought stress tolerance in poplars (Populus) by modification of candidate genes. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 35
- 22
Fendel A, Fladung M, Brügmann T (2022) Improvement of drought stress tolerance in poplars (Populus) by modification of candidate genes. In: Plant Sciences for a Sustainable Future : Botanik-Tagung, International Conference of the German Society for Plant Sciences, 2022 Bonn, 28 August - 01 September ; Programme. Deutsche Botanische Gesellschaft, p 104
- 23
Brügmann T, Biricolti S, Becker D, Fladung M (2022) On track towards DNA-free genome editing. In: COST Action CA18111 "Genome Editing in Plants" : Book of Abstracts ; 3rd PlantEd Conference, 5 - 7 September 2022, Düsseldorf, Germany. p 49
- 24
Brügmann T, Deecke K, Fladung M (2019) Evaluating the efficiency of gRNAs in CRISPR/Cas9 mediated genome editing in poplars. Int J Mol Sci 20(15):3623, DOI:10.3390/ijms20153623
- 25
Brügmann T, Fladung M (2019) Genom-Editierung in Bäumen. AFZ Der Wald 74(5):16-18
- 26
Brügmann T, Wetzel H, Hettrich K, Smeds A, Willför S, Kersten B, Fladung M (2019) Knockdown of PCBER1, a gene of neolignan biosynthesis, resulted in increased poplar growth. Planta 249(2):515-525, DOI:10.1007/s00425-018-3021-8
- 27
Brügmann T, Fladung M (2019) Overexpression of both flowering time genes AtSOC1 and SaFUL revealed huge influence onto plant habitus in poplar. Tree Genetics Genomes 15:20, DOI:10.1007/s11295-019-1326-9
- 28
Brügmann T, Polak O, Deecke K, Nietsch J, Fladung M (2019) Poplar transformation. Meth Mol Biol 1864:165-177
- 29
Brügmann T, Fladung M (2016) Flowering time genes influence biomass production in poplars. In: Hensel G (ed) Annual meeting : new breeding technologies in times of politically enforces research prohibition ; German Society of Plant Biotechnology e.V. ; book of abstracts ; Gatersleben, 02.-04.05.2016. Gatersleben: Gesellschaft für Pflanzenbiotechnologie, p 23
- 30
Brügmann T, Polak O, Fladung M (2015) Development and use of novel gene technologies to increase biomass yield in the woody perennial Populus spec. (PopMass) : project A - modification of genes expressed in xylem and flower. In: Conference documents Plant 2040 Status Seminar, March 4 - 6, 2015 in Potsdam. pp 67-68
- 31
Brügmann T, Fladung M (2015) Genetic approaches to increase biomass yield in the woody perennial Populus. In: Vettori C, Vendramin GG, Paffetti D, Travaglini D (eds) IUFRO Tree Biotechnology 2015 Conference : "Forests: the importance to the planet and society" ; 8-12 June 2015, Florence, Italy ; Proceedings. Florenz: IUFRO, pp 507-509
- 32
Schröder H, Brügmann T, Fladung M (2015) Nuclear and chloroplast SNP markers support successful poplar breeding. In: Vettori C, Vendramin GG, Paffetti D, Travaglini D (eds) IUFRO Tree Biotechnology 2015 Conference : "Forests: the importance to the planet and society" ; 8-12 June 2015, Florence, Italy ; Proceedings. Florenz: IUFRO, pp 295-296
- 33
Hönicka H, Fladung M, Lehnhardt D, Brügmann T (2013) Pleiotropic effects of "flowering" genes in transgenic poplar. In: SPP1530 workshop: Pleiotropic effects of flowering time genes and impact on adaptation and speciation, January 21-23,2013, Max Planck Institute for Plant Breeding Research, Köln. p 13
- 34
Brügmann T, Fladung M (2013) Potentials and limitations of the cross-species transfer of nuclear microsatellite marker in six species belonging to three sections of the genus PopulusL.. Tree Genetics Genomes 9(6):1413-1421, DOI:10.1007/s11295-013-0647-3
- 35
Brügmann T (2011) Entwicklung molekularer Marker zur genetischen Charakterisierung von Genotypen verschiedener Arten der Gattung Populus L.. Hamburg: Universität, 134 p, Hamburg, Univ, Fachber Biologie, Masterarbeit, 2011
- 36
Brügmann T (2009) Optimierung von RNA-Extraktion und cDNA-Synthese aus Quercus robur. Hamburg: Univ, 76 p, Hamburg, Univ, Department Biologie, Bachelorarbeit, 2009
