Biosafety of transgenic trees

Project

Gene transfer and biosafety of biotechnologically grown trees

Following the year 2008 transgenic trees to be foreseen for non-commercial field releases as well as for commercial applications shouldn't contain any antibiotic resistance gene marker. Further, it has been claimed in particular for trees that the foreign genes introduced into tree genomes should be positioned at genomic locations which has been found suitable for integration of forest genes. In this project the possibilities for a targeted transfer of transgenes as well the elimination of marke

Background and Objective

In this project the possibilities for a targeted transfer of transgenes as well the elimination of marker genes will be studied. In frame of a biological biosafety research are these investigations very important in order to assess possible risks following the practical application of such systems.

Approach

This project is based on preliminary with using recombination systems, which has been successfully tested in annual plants. Also for aspen it has been shown, that the recombination systems Cre/lox as well FLP/FRT work well. For risk assessment studies it has to be analysed whether pleiotropic effects can be observed in transgenic trees carrying recombination systems.

Preliminary Results

This project is based on preliminary with using recombination systems, which has been successfully tested in annual plants. Also for aspen it has been shown, that the recombination systems Cre/lox as well FLP/FRT work well. For risk assessment studies it has to be analysed whether pleiotropic effects can be observed in transgenic trees carrying recombination systems.

Thünen-Contact

Fladung, Matthias Institute of Forest Genetics

Involved Thünen-Partners

Duration

3.2004 - 5.2024

More Information

Projekt type:
Project status: ongoing

Publications

Results 1 - 10 of 16

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Vettori C, Fladung M (2016) Introduction. Forestry Sci 82:1-7
Pilate G, Allona I, Boerjan W, Dejardin A, Fladung M, Gallardo F, Häggman H, Jansson S, van Acker R, Halpin C (2016) Lessons from 25 years of GM tree field trials in Europe and prospects for the future. Forestry Sci 82:67-100, DOI:10.1007/978-94-017-7531-1_4
Biricolti S, Bartsch D, Boerjan W, Fladung M, Glandorf DCM, Sweet JB, Gallardo F (2016) Potential impacts of GM trees on the environment and on plant "Omics": questionnaire-based responses. Forestry Sci 82:195-205
Kazana V, Tsourgiannis L, Iakovoglou V, Stamatiou C, Alexandrov A, Bogdan S, Bozic G, Brus R, Bossinger G, Boutsimea A, Celepirovic N, Cvrcková H, Fladung M, et al (2016) Public attitudes towards the use of transgenic forest trees: a crosscountry pilot survey. iForest 9(2):344-353, DOI:10.3832/ifor1441-008
Kazana V, Tsourgiannis L, Iakovoglou V, Fladung M, et al (2016) Public knowledge and perceptions of safety issues towards the use of genetically modified forest trees: a cross-country pilot survey. Forestry Sci 82:223-244
Gallardo F, Sánchez C, Grabowski M, Molina-Rueda JJ, Vidal N, Fladung M (2016) Soil effects of genetically modified trees (GMTs). Forestry Sci 82:155-172
Häggman H, Sutela S, Walter C, Fladung M (2014) Biosafety considerations in the context of deployment of GE trees. Forestry Sci 81:491-524, doi:10.1007/978-94-007-7076-8_21
Vettori C, Pilate G, Häggman H, Gallardo F, Ionita L, Ruohonen-Lehto M, Glandorf B, Harfouche A, Biricolti S, Paffetti D, Kazana V, Sijacic-Nikolic M, Tsourgiannis L, Migliacci F, Donnarumma F, Minol K, Fladung M (2014) COST Action FP0905: biosafety of forest transgenic trees. In: Ramawat KG, Mérillon J-M, Ahuja MR (eds) Tree biotechnology. Boca Raton: CRC Press ; Taylor & Francis, pp 112-124
Ahuja MR, Fladung M (2014) Integration and inheritance of transgenes in crop plants and trees. Tree Genetics Genomes 10(4):779-790, DOI:10.1007/s11295-014-0724-2
Kersten B, Pakull B, Groppe K, Lüneburg J, Fladung M (2014) The sex-linked region in Populus tremuloides Turesson 141 corresponds to a pericentromeric region of about two million base pairs on P. trichocarpa chromosome 19. Plant Biol 16(2):411-418, DOI:10.1111/plb.12048