Activation tagging 2
Molecular Analysis of activation-tagged Aspen-Populus Variants II
So far, only very few naturally available tree mutants are known, most of them showing alterations in tree habitus (e.g. dwarf, ‘columnar’, ‘pendula’ mutants) and/or variations in leaf form/shape/color (e.g. ‘lanceolate’, ‘brevifolia’, and pale green leaf mutants). In a preceding proposal, the fundamentals for transposon (Ac from maize)-based activation tagging in a tree species (Populus spec.) was developed.
Background and Objective
Proof-of-concept for using activation tagging to generate poplar mutants was provided. In this proposal, already available mutants will be molecular analysed and new mutants will be generated by applying a highly efficient mutant induction system.
A number of highly innovative aspects are considered, namely (a) due to the low number of naturally available tree mutants, it is the first attempt to establish an efficient system to systematically generate mutants in poplar, a tree species, (b) with exception of Arabidopsis, rice and few others, it is novel to combine T-DNA and transposon activation tagging approaches, (c) it is a challenge to proof the involvement of the tagged (candidate) gene in a tree species by a transformation approach, (d) to develop a phenotyping strategy for activation tagged poplar plants, and (e) it is highly interesting to test different possibilities for the storage of mutant tissue (incl. cryopreservation) of a tree species.
Fladung M (2014) Prospects of using a modified Ac/Ds transposon system from maize for activation tagging in the tree species Populus. In: Ramawat KG, Mérillon J-M, Ahuja MR (eds) Tree biotechnology. Boca Raton: CRC Press ; Taylor & Francis, pp 469-482
Fladung M, Polak O (2012) Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery. BMC Genomics 13(61), DOI:10.1186/1471-2164-13-61
Fladung M, Polak O (2011) Activation tagging in poplar by using an inducible Ac/Ds transposon system. BMC Proc 5(Suppl. 7):O 51, DOI:10.1186/1753-6561-5-S7-O51