Head of Unit

PD Dr. Matthias Fladung

Sieker Landstraße 2

22927 Großhansdorf

Telefon +49 4102 696 107

Fax +49 4102 696 200

e-Mail matthias.fladung@  thuenen.de

Transformation-induced mutations

Project

 (c)

Transformation-induced mutations in biotechnologically produced trees

Genetically transferred DNA as well as genome editing methiods (CRISPR/Cas) are putative causal agents for genomic instabilities.

Background and Objective

For regulation of the transferred foreign genes the 35S promoter of the cauliflower mosaic virus is used very frequently.

In the research project, potential biosafety related risks will be evaluated resulting from the interactions between the already mentioned 35S-promoter (as well as other sequences from viruses) on one hand, and poplar viruses and retro elements on the other hand. These interactions could either activate so far “sleeping” retro elements or let evolve novel retro elements following recombination.

In addition, the complete genome of plants modified by CRISPR/Cas9 will be sequenced and investigated for possible off-target mutations.

Approach

In initiating experiments already existing transgenic poplar lines were investigated in respect to genomic stability using the AFLP technique.

Meanwhile, next-generation sequencing methods are being used.

Preliminary Results

The results indicate that as expected a high genetic stability is existing between different transgenic lines and their respective non-transgenic controls. However, single cases have been observed where reproducible differences were detected in the AFLP pattern which indicate a transformation-induced genomic mutation. Further investigations are planned to unravel the basis for the observed variations.

Thünen-Contact


Involved Thünen-Partners


Duration

Permanent task 1.2005 - 5.2024

More Information

Projekt type:
Project status: ongoing

Publications

hits: 2

  1. Fladung M (2016) Cibus' herbicide-resistant canola in European limbo. Nature Biotechnol 34(5):473-474
  2. Fladung M (2014) Prospects of using a modified Ac/Ds transposon system from maize for activation tagging in the tree species Populus. In: Ramawat KG, Mérillon J-M, Ahuja MR (eds) Tree biotechnology. Boca Raton: CRC Press ; Taylor & Francis, pp 469-482