Institut für Fischereiökologie
Phone: +49 40 38905 290
Fax: +49 40 38905 261
The zebrafish (Danio rerio) is one of the favourite organisms for developmental biologists and for toxicologists.The embryonal stage of this fish is completed in a few days and can be observed easyly due to the transparent chorion.
The early life stage test with zebrafish eggs can help to assess toxic impact of pollutants by orbservation of developmental abberations in the embryo. After 48h of embryonal development the test is terminated. The same embryos can be used for gene expression studies or other effect specific methods.
Briefly, fertilized and well-developed eggs in the 4 to 8 cell stage were selected for the test and placed in 24-well dishes. In each well 5 eggs were exposed in 1 mL test solution containing DMSO and water. 60 eggs were used for each sample (known concentration of contaminant, positive or negative control). Incubation took place at 26°C for 48 h.
After that Eggs were monitored for coagulation and malformations using an inverse microscope. Lethal (coagulation, missing heartbeat, missing somites, missing tail detachment from the yolk sac, missing spontaneous movement) and non-lethal malformations (yolk sac edema, spine deformation, no pigmentation, incomplete eye development) were reported separately. The test ist repeated twice
Results are presented as dose-response curves for calculation of the desired threshold levels such as EC10 or EC50. All lethal an non-lethal deformations are reported. Some alterations allow insight in the mode of Action of the chemical.
Numerous results obtained with the fish embryo test investigating single substances as well as environmental extracts have been published as listed below.
Permanent task 1.2001 - 12.2020
Project status: ongoing
Results 1 - 5 of 7
Results 1 - 5 of 7