Genome analysis in trees

Project

 (c)

Structural and functional analysis of genomes of forest trees

Trees are characterized by a number of specific characteristics which are under genetic control, like longevity and wood formation.

Background and Objective

It is expected that several genes are involved in the mentioned processes. The aim of this task is the isolation and characterization of regulatory and other different gene sequences which are specific for trees. Species of the genus Populus (poplar) will be used for the investigations.

Approach

Poplars are an excepted model system for trees because of their relatively small genome and accessibility to the Agrobacterium transformation system. Following Arabidopsis and rice the genome of Populus has been totally sequenced. These information serve as basis for the planned investigations.

Preliminary Results

The strategy is based on results of earlier work. Here it was shown for the first time that the genetic transformation of poplar using the transposon Ac from maize is feasible. The results show that Ac excises from its original position in the construct and re-integrates somewhere in the genome (transposition). Further it was demonstrated, that transposition of Ac still occurs in six-years old transgenic plants.; ; Following transformation of Ac more than 100 different, independent transgenic aspe

Thünen-Contact


Involved Thünen-Partners


Duration

Permanent task 2.2000 - 5.2024

More Information

Projekt type:
Project status: ongoing

Publications

Results 1 - 10 of 21

Begin   back   next   end

  1. Hernandez-Velasco J, Hernández-Diaz JC, Fladung M, Cañadas-Lopez A, Prieto-Ruiz JA, Wehenkel C (2017) Spatial genetic structure in four Pinus species in the Sierra Madre Occidental, Durango, Mexico. Can J Forest Res 47:73-80, DOI:10.1139/cjfr-2016-0154
  2. Ortiz-Olivas ME, Hernández-Diaz JC, Fladung M, Cañadas-Lopez A, Prieto-Ruiz JA, Wehenkel C (2017) Spatial genetic structure within and among seed stands of Pinus engelmannii Carr. and Pinus leiophylla Schiede ex Schltdl. & Cham, in Durango, Mexico. Forests 8:22, DOI:10.3390/f8010022
    pdf document (limited accessibility) 1985 kb
  3. Kersten B, Faivre Rampant P, Mader M, Le Paslier M-C, Bounon R, Berard A, Vettori C, Schröder H, Leplé J-C, Fladung M (2016) Genome sequences of Populus tremula chloroplast and mitochondrion: Implications for holistic poplar breeding. PLoS One 11(1):e0147209, DOI:10.1371/journal.pone.0147209
    pdf document (limited accessibility) 1192 kb
  4. Pakull B, Kersten B, Lüneburg J, Fladung M (2015) A simple PCR-based marker to determine sex in aspen. Plant Biol 17(1):256-261, DOI:10.1111/plb.12217
  5. Fladung M (2014) Prospects of using a modified Ac/Ds transposon system from maize for activation tagging in the tree species Populus. In: Ramawat KG, Mérillon J-M, Ahuja MR (eds) Tree biotechnology. Boca Raton: CRC Press ; Taylor & Francis, pp 469-482
  6. Kersten B, Pakull B, Groppe K, Lüneburg J, Fladung M (2014) The sex-linked region in Populus tremuloides Turesson 141 corresponds to a pericentromeric region of about two million base pairs on P. trichocarpa chromosome 19. Plant Biol 16(2):411-418, DOI:10.1111/plb.12048
  7. Fladung M (2013) Efficient in vitro plantlet regeneration in Populus euphrata Oliver [online]. Afr J Biotechnol 12(8):826-832, zu finden in <http://www.academicjournals.org/journal/AJB/article-full-text-pdf/81F997626646> [zitiert am 12.11.2013]
  8. Fladung M, Gebhardt K, Kersten B (2013) Erbgut verschiedener Nadelbäume vollständig entschlüsselt. AFZ Wald 68(20):13-15
    pdf document (limited accessibility) 242 kb
  9. Fladung M, Hönicka H, Ahuja MR (2013) Genomic stability and long-term transgene expression in poplar. Transgenic Res 22(6):1167-1178, DOI:10.1007/s11248-013-9719-2
  10. Bubner B, Fladung M, Lentzsch P, Münzenberger B, Hüttl RF (2013) Individual tree genotypes do not explain ectomycorrhizal biodiversity in soil cores of a pure stand of beech (Fagus sylvatica L.). Trees 27(5):1327-1338, DOI:10.1007/s00468-013-0881-1

Results 1 - 10 of 21

Begin   back   next   end